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Immunohistochemical detection of c‐Ha‐ ras oncogene p21 product in pre‐neoplastic and neoplastic lesions during hepatocarcinogenesis in rats
Author(s) -
Galand P.,
Jacobovitz D.,
Alexandre K.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410127
Subject(s) - immunohistochemistry , pathology , oncogene , cancer research , neoplastic disease , biology , cancer , medicine , cell cycle , genetics
An immunohistochemical study of c‐Ha‐ ras expression was performed on preneoplastic and neoplastic stages of diethl nitrosamine (DENA)‐induced hepatocarcinogenesis in rats, using an antibody raised against a peptide sequence of the Ha‐ ras p21 product. Moderate to high immunostaining intensity was observed in the following hepatocytic lesions: (I) hepato‐cellular carcinomas (14/14) and associated neoplastic nodules (S/S) and foci of phenotypic alterations (35/40) (after 13–20 months of treatment); (2) neoplastic nodules (6/6) and associated foci (42/50) (after 5–9 months); (3) foci (10/10) (after 2 months); and (4) small, slowly growing foci (26/40) found 9 months after treatment with DENA without prior partial hepatectomy, resulting in low number of nodules and no tumor even after IS months. No c‐Ha‐ ras p21 was detected immunohistochemically in normal nor in regenerating rat liver. Our result indicate that increased Ha‐ ras expression is an early and stable event in liver lesions associated with he‐patocarcinogenesis. They also imply that increased Ha‐ ras expression is insufficient (if at all implicated) for inducing fully malignant hepatocyte transformation. It might be indicative of cell populations at an increased transformation risk.