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Metastasis of a human melanoma cell line in the nude mouse
Author(s) -
Wilson E. Lynette,
Gartner Maria F. R. M.,
Campbell J. A. H.,
Dowdle Eugene B.
Publication year - 1988
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910410116
Subject(s) - metastasis , melanoma , autopsy , pathology , medicine , metastatic melanoma , biology , cancer research , cancer
A human melanoma cell tine has been established which when inoculated subcutaneously into nude mice, is consistently metastatic. In order to document blood‐borne spread, it was necessary to excise the primary tumour so prolonging the life of the animal and allowing metastases to become apparent. Macroscopicatly detectable metastatic spread at autopsy was reliably indicated by weight loss of the animals. Metastases were widespread and involved the lungs, abdominal cavity and organs and the gonads. The size of the primary tumour at the time of its removal, and nat the period of s.c. growth, determined the incidence of metastatic disease. Removal of tumours weighing less than 0.6 g prevented metastasis, whereas all of the animals showed widely disseminated disease if the tumour was allowed to attain a size of 1.6 g before excision.