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Analysis of the chromosomal abnormalities of a woodchuck hepatocellular carcinoma‐derived cell line (WH257GE10)
Author(s) -
Matsushita Fumiaki,
Ohno Shinsuke,
Unoura Masashi,
Kobayashi Kenichi,
Tanaka Nobuyoshi,
Fukuoka Kenichi,
Morioka Takeshi,
Kaneko Shuichi,
Aoyama Syou,
Migita Shunsuke,
Hattori Nobu
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910400524
Subject(s) - karyotype , autosome , biology , chromosome , genetics , woodchuck hepatitis virus , microbiology and biotechnology , chromosome 16 , chromosome 3 , marker chromosome , cytogenetics , x chromosome , gene , virus , hepatitis b virus , hepadnaviridae
The normal woodchuck karyotype was determined by the G‐banding technique, and the chromosomal abnormalities of a woodchuck hepatocellular carcinoma‐derived cell line (WH257GE10) were analyzed and compared with it. The normal woodchuck karyotype was 38, XY in a male, and 38, XX in a female. Autosomes were classified into 2 groups (A and B). Group A consisted of 13 pairs (1–13) of metacentric or submetacentric chromosomes, and group B consisted of 5 pairs (14–18) of telocentric or sub‐telocentric ones. The X chromosome was sub‐metacentric, and the Y chromosome was telocentric. In WH257GE10, the modal number was 36. Deletion of one of the chromosome 5 pair, of the chromosome 13 pair, and of the Y chromosome was observed. Two marker chromosomes (M 1 and M 2 ) were found. These markers showed the same G‐banded patterns except for a small chromosome fragment at the distal part of the long arm of M 1 . The chromosome aberrations were very stable through successive passages in vitro. These findings suggest that the chromosomal abnormalities found in WH257GE10 might be associated with the pathogenesis of woodchuck hepatocellular carcinoma.