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A cell line from Wilms' tumour with deletion in short arm of chromosome 11
Author(s) -
Kumar S.,
Harrison Christine J.,
Heighway J.,
Marsden H. B.,
West D. C.,
Jones Pat Morris
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910400412
Subject(s) - wilms tumour , long arm , chromosome , wilms' tumor , biology , karyotype , genetics , medicine , cancer research , gene
A cell line (T3/73) from a Wilms' tumour has been established from a 9‐month‐old boy with aniridia. The tumour was removed in 1973. On histological examination a diagnosis of Wilms' tumour was made which showed undifferentiated areas, marked tubule formation and abundant striped muscle fibers. The tumour cells, which are fusiform, grew rapidly in culture without the addition of growth factors, and have undergone over 100 passages. Approximately 95% and 5% were positive for desmin and cytokeratin, respectively. The cell doubling time was 28 hr. Cytogenetic studies revealed a karyotype of 46, XY, del(11) (p12::p14). Although the cells stained very intensely with a monoclonal antibody that detects oncogene ras p 21 antigen, Southern blot analysis using c‐Ha‐ ras as a probe failed to reveal an obvious deletion or amplification of either Ha‐ ras allele.