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Spontaneous mutation rates in cloned murine tumors do not correlate with metastatic potential, whereas the prevalence of karyotypic abnormalities in the parental tumors does
Author(s) -
Kendal Wayne S.,
Wang RuiYu,
Frost Philip
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910400321
Subject(s) - biology , mutation , cancer research , genome instability , cancer , cell culture , ouabain , chromosome instability , mutation rate , genetics , pathology , medicine , chromosome , gene , dna , dna damage , chemistry , organic chemistry , sodium
We tested the hypothesis that highly malignant cell lines are genomically more unstable than their less malignant counterparts, and that this instability is more pronounced in clones than in cell lines. We compared MDAY‐D2 to its non‐metastatic variant, D36W25, with regard to (I) the rate of development of ouabain resistance within parallel clones and (2) the prevalence of G‐banded karyotypic abnormalities. We detected no significant difference between the spontaneous mutation rates for ouabain resistance. However, the MDAY‐D2 cell line possessed both a higher prevalence and greater diversity of chromosomal abnormalities. One possible explanation for these seemingly inconsistent results is that genomic instability may remain essentially constant throughout tumor progression, whereas an accumulation of genetic changes may be responsible for the observed increased prevalence of abnormalities and the development of selective survival advantages during progression.