Premium
Suppression of NK activity of spleen cells by chicken fetal antigen present on Marek's disease lymphoblastoid cell line cells
Author(s) -
Ohashi Kazuhiko,
Mikami Takeshi,
Kodama Hiroshi,
Izawa Hisao
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910400316
Subject(s) - spleen , antigen , ovalbumin , marek's disease , lymphoblast , biology , monoclonal antibody , immune system , cell culture , antibody , microbiology and biotechnology , immunology , virology , virus , genetics
Abstract Chicken fetal antigen (CFA) expressed on the cell surface of Marek's disease (MD) lymphoblastoid cell line (MDCC‐MSBI) cells was purified by affinity chromatography using a monoclonal antibody (2H3). A 4‐hr 51 Cr release assay was performed using spleen cells as effector cells to investigate the role of the CFA in the immune response of chickens, especially in relation to natural killer (NK) activity. Spleen cells of specific‐pathogen‐free (SPF) chickens showed reduced NK activity in the presence of CFA in vitro , as did those cells treated with MSBI soluble antigen. NK activity of spleen cells from chickens treated with CFA was also suppressed when compared to the cells from untreated or ovalbumin‐treated chickens. In addition, MSBI clo. 18 transplantable tumors grew progressively in some of the 14‐day‐old chickens treated with CFA, whereas the tumors regressed in age‐matched chickens with or without treatment by ovalbumin.