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Conversion of the lymphoma line “BJAB” by Epstein‐Barr virus into phenotypically altered sublines is accompanied by increased C‐ myc mRNA levels
Author(s) -
Wennborg Anders,
Åman Pierre,
Saranath Dhananjaya,
Pear Warren,
Sümegi Janos,
Klein George
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910400213
Subject(s) - lymphoma , chromosomal translocation , biology , cell culture , epstein–barr virus , virus , oncogene , microbiology and biotechnology , messenger rna , virology , transfection , cancer research , gene , immunology , genetics , cell cycle
The steady‐state level of c‐ myc proto‐oncogene mRNA was investigated in the EBV‐negative human B‐lymphoma line BJAB and 2 sublines that have been converted by EBV into stable EBV‐genome‐carrying and EBNA‐positive status. The EBV‐converted sublines expressed c‐ myc at a 2‐to 6‐fold higher level than the original BJAB during exponential growth. The EBV‐positive BJAB lines are known to differ from the parent line in several phenotypic characteristics, including increased agarose clonability, lower serum requirements and, in one case, increased tumorigenicity in nude mice. The pattern of increased c‐ myc expression accompanying EBV conversion was not observed in the myc /lg translocation‐carrying Burkitt lymphoma line RAMOS or in 2 of its EBV‐converted sublines.