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Amplification of the epidermal growth factor receptor gene in the BT20 breast carcinoma cell line
Author(s) -
Lebeau Jérome,
Goubin Gérard
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910400210
Subject(s) - biology , northern blot , southern blot , cell culture , epidermal growth factor , a431 cells , microbiology and biotechnology , epidermal growth factor receptor , mammary gland , gene duplication , gene , cancer research , receptor , gene expression , genetics , cell cycle , cancer , breast cancer , molecular medicine
Southern blot‐hybridization analysis of 10 breast carcinoma cell lines demonstrated an amplification of the epidermal growth factor (EGF) receptor gene in the BT20 cell line. The gene was amplified 10‐to 14‐fold when compared to the level seen in human placental DNA. Northern blot analysis indicated an increased level of transcription in this cell line which probably accounts for the high level of the EGF receptor reported in the BT20 cell line.