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Interrelationships among X‐ray‐induced anchorage independence, mutagenesis and chromosomal rearrangements in human diploid fibroblasts
Author(s) -
Kano Yoshio,
Grosovsky Andrew J.,
Little John B.
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910400112
Subject(s) - ploidy , mutagenesis , genetics , independence (probability theory) , biology , mutation , microbiology and biotechnology , gene , statistics , mathematics
The changes in the frequencies of X‐ray‐induced mutations to 6‐thioguanine resistance, chromosomal abnormalities and transformation to anchorage‐independent growth were examined during confluent holding recovery in density‐inhibited cultures of human diploid fibroblasts. Chromosomal abnormalities studied included non‐stable aberrations, deletions, and reciprocal translocations measured up to 20 mean population doublings post‐irradiation. Complex recovery kinetics were observed. A comparison of these kinetics suggests that reciprocal translocations are associated with the induction of both transformation and mutations. Mutagenesis correlated with deletions and translocations, whereas transformation correlated with translocations only.