Premium
Glycoproteic nature of surface molecules of effector cells with lymphokine‐activated killer (LAK) activity. Evidence that T11, T8 or T3 molecules are not involved in tumor‐cell lysis by LAK effector T cells
Author(s) -
Bagnasco Marcello,
Mingari Maria Cristina,
Pende Daniela,
Venuti Daniela,
Caica Giorgio Walter,
Moretta Lorenzo
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910390608
Subject(s) - lymphokine activated killer cell , effector , interleukin 2 , population , microbiology and biotechnology , biology , tunicamycin , monoclonal antibody , lymphokine , cytolysis , chemistry , interleukin 21 , cytotoxic t cell , immunology , antibody , cytokine , biochemistry , in vitro , antigen , medicine , apoptosis , environmental health , unfolded protein response
Human peripheral blood mononuclear cells cultured in the presence of interleukin‐2 (IL‐2) acquire the capability of lysing NK‐resistant fresh tumor target cells. In an attempt to delineate the surface structure(s) present on the effector cells, the latter were first treated with different amounts of pronase and neuraminidase. The effect of the enzymes on cytolytic activity against fresh melanoma cells was evaluated and compared with the NK‐like activity against K562 target cells of the same effector population. At a pronase concentration of 0.01 mg/ml, no inhibition of NK‐like activity was detected, whereas LAK activity was inhibited by more than 75%. In addition, neuraminidase had no effect on NK‐like activity, even at I U/ml, whereas as little as 0.03 U/ml inhibited LAK activity by more than 75%. Metabolic inhibition of N‐linked glycosylation with Tunicamycin prevented the generation of LAK activity, even when added late (18 hr before termination of the culture). Tunicamycin, on the other hand, had no effect on the boost of NK activity induced by IL‐2. Provided that LAK activity can also be generated in T‐cell (E‐rosetting) populations, in the presence of adherent cells, we analyzed the inhibitory activity of monoclonal antibodies (MAbs) to T11, T3 and T8 molecules. While all these MAbs strongly inhibited the specific target cell lysis by alloreactive CTLs, they had no effect on the LAK activity.