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Cellular heterogeneity and drug resistance in two ovarian adenocarcinoma cell lines derived from a single patient
Author(s) -
Wolf C. Roland,
Hayward Ian P.,
Lawrie Sandra S.,
Buckton Karin,
McIntyre Margaret A.,
Adams David J.,
Lewis Alexander D.,
Scott Angela R. R.,
Smyth John F.
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910390607
Subject(s) - karyotype , biology , cell culture , cancer research , chlorambucil , chemotherapy , population , adenocarcinoma , genetics , oncology , chromosome , medicine , endocrinology , cancer , gene , environmental health , cyclophosphamide
Abstract Two ovarian cell lines were derived from the ascites of a patient before and after the onset of resistance to chemotherapy involving cis‐platinum, chlorambucil and 5‐fluorouracil. Characterization of these lines shows them to have various features in common and some significant differences. Cytologically the lines cannot be distinguished and they both contain high concentrations of oestrogen receptor. However, they do differ with respect to their growth characteristics, karyotype, glutathione content and sensitivity to cis‐platinum. The karyotypes of the 2 lines show several marker chromosomes in common but the resistant line contained a chromosome 8 and a 17 which were absent from the earlier sensitive line. This suggests a clonal origin with subsequent divergence to a heterogeneous population.