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Structure and expression of mos sequences in spontaneous and moloney murine sarcoma virus‐induced yolk sac carcinomas in rats
Author(s) -
Decock B.,
Sobis H.,
Van Hove L.,
Vandeputte M.,
Billiau A.
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910390418
Subject(s) - provirus , murine leukemia virus , biology , virus , yolk sac , virology , microbiology and biotechnology , cell culture , southern blot , northern blot , rna , dna , genome , embryo , gene , genetics
Facilitation of yolk‐sac carcinoma (YSCa) development in fetectomized rats by the Moloney murine sarcoma virus/murine leukemia virus (Mo‐MSV/MLV) complex was found to be closely associated with the presence of Mo‐MSV sequences in the genomes of the YSCa cells. The virus‐induced YSCas consisted of cells of mono‐ or oligoclonal origin which always contained in their genomes at least 1 randomly integrated Mo‐MSV provirus. In YSCas which developed in the absence of virus, no rearrangement or amplification of c‐ mos could be detected. In addition, blot hybridization analysis of cellular RNA failed to detect mos ‐related RNA in cell lines derived from Mo‐MSV‐induced as well as from non‐virally induced YSCas. The methylation level of c‐ mos DNA was low in all YSCa cell lines. In contrast, v‐ mos DNA in cell lines derived from Mo‐MSV‐induced YSCas was heavily methylated.