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Transformation‐related changes in the expression of endogenous cell lectins
Author(s) -
Raz Avraham,
Meromsky Leonid,
Zvibel Isabel,
Lotan Reuben
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910390314
Subject(s) - endogeny , transformation (genetics) , expression (computer science) , biology , cell , microbiology and biotechnology , immunology , biochemistry , computer science , gene , programming language
Endogenous lectins purified from UV‐2237‐IP3 murine fibrosarcoma cells by affinity chromatography consisted of two polypeptide species of M r 14,500 (L‐14.5) and M r 34,000 (L‐34). Antibodies against this material immunoprecipitated the lectins from cells radiolabelled on the cell surface as well as in the cytoplasmic compartment. Similar analyses of normal rat embryonal fibroblasts revealed the presence of only the L‐14.5. In contrast, both L‐14.5 and L‐34 were found in oncogenetransfected, immortalized cell clones derived from the normal rat cells, as well as in untransformed BALB/c‐3T3 clone A31 and its descendant subclones selected for expression of the transformed, the tumorigenic and the metastatic phenotypes. Among the cells constituting the latter system, a marked increase in the amount of cellular and cell‐surface lectins was observed upon progression to the metastatic phenotype. These results suggest that the expression of endogenous tumor‐cell‐surface lectins is associated with transformation and metastasis.