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Effects of DL‐α‐difluoromethylornithine on the growth and metastasis of b16 melanoma in vivo
Author(s) -
Kubota Shunichiro,
Ohsawa Nakaaki,
Takaku Fumimaro
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910390220
Subject(s) - ornithine decarboxylase , melanoma , in vivo , spermidine , putrescine , eflornithine , metastasis , medicine , cancer research , immunology , biology , cancer , enzyme , biochemistry , microbiology and biotechnology
The effects of DL‐α‐difluoromethylornithine (DFMO), a specific irreversible inhibitor of ornithine decarboxylase, on the growth of experimental mouse B16‐F10 melanoma cells were investigated. DFMO (3%) in drinking water was administered to B16‐F10 melanoma‐bearing mice. At 24 days, B16‐F10 melanomas in DFMO‐fed mice weighed 75% less than those in control mice ( p < 0.001). DFMO reduced putrescine and spermidine levels in B16‐F10 melanoma by 98% and 84%, respectively, and prolonged the mean survival time from 25.9 ± 1.2 to 35.7 ± 2.2 days ( p < 0.001). The effects of DFMO on experimental metastasis were also investigated. DFMO treatment resulted in a significant decrease in pulmonary metastasis induced by i.v. injection of B16‐F10 melanoma cells.

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