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Reactive T cells in the immune repertoire: Self‐restricted and allo‐restricted helper T‐cell clones to epstein‐barr virus
Author(s) -
Lai Patrick K.,
Pauza Mary E.,
Switzer Barbara L.,
Smith Douglas,
Purtilo David T.
Publication year - 1987
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910390120
Subject(s) - clone (java method) , biology , t cell , epstein–barr virus , virology , cytotoxic t cell , antigen , monoclonal antibody , t lymphocyte , human leukocyte antigen , immunology , t helper cell , streptamer , microbiology and biotechnology , virus , antibody , interleukin 21 , immune system , genetics , in vitro , dna
Helper T‐cell clones were generated by stimulation with autologous or allogeneic lymphoblastoid B cells (B‐LCL) transformed by the Epstein‐Barr virus (EBV). Some of these T‐cell clones were allo‐reactive and others were specific to EBV‐transformed B‐LCL. Helper T‐cell clones specific to EBV‐transformed B‐LCL were restricted either by class‐1 or by class‐11 HLA molecules of self. T‐cell clones restricted by class‐1 HLA molecules were stained by OKT3 and OKT8 monoclonal antibodies (MAbs), whereas class‐11‐restricted clones stained with OKT3 and OKT4. Not all helper T‐cell clones specific to EBV‐transformed B‐LCL were restricted to self: one clone restricted by allo‐HLA antigen was established. This finding suggests that in humans, as in mice, some T cells in the T‐cell repertoire can be allo‐restricted. This allo restriction may represent cross‐reactivity of T cells, whereby “self + X” equals “allo + Y.” Activation of these cross‐reacting T cells restricted by allogeneic HLA molecules during infectious mononucleosis will give a T‐cell response which may appear unrestricted by self HLA molecules. This mechanism helps to explain, at least in part, the HLA unrestricted cytotoxicity to B‐LCL observed in infectious mononucleosis.