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Experimental inoculation of monkeys with autologous lymphoid cell lines immortalized by and producing human T‐cell leukemia virus type‐I
Author(s) -
Nakamura Hideo,
Tanaka Yuetsu,
KomuroTsujimoto Atsumi,
Ishikawa Kohichi,
Takadaya Kumiko Iijima,
Tozawa Hideki,
Tsujimoto Hajime,
Honjo Shigeo,
Hayami Masanori
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910380614
Subject(s) - virology , inoculation , leukemia , cell culture , biology , virus , cell , immunology , pathology , medicine , genetics
Cynomolgus monkeys and squirrel monkeys were inoculated with autologous lymphoid cell lines immortalized by and producing human T‐cell leukemia virus type‐1 (HTLV‐I) in order to serve as an animal model of adult T‐cell leukemia (ATL). The autologous cell tines were established from peripheral blood mononuclear cells (PBMC) from each monkey by co‐cultivation with lethally irradiated MT‐2 cells producing HTLV‐I. All of these cell lines, which had monkey karyotypes, grew continuously without addition of interleukin‐2 (IL‐2) and expressed virus‐specific proteins of HTLV‐I and IL‐2 receptor. After inoculation with the autologous cell lines, specific antibodies against HTLV‐I proteins could be detected in their plasma, and transformed HTLV‐I‐infected cells could be recovered from their peripheral blood for at least 6 months. However, no signs of ATL have been observed to date, i.e. 2 years after inoculation.