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Plant‐derived diterpene esters enhance HTLV‐I‐induced colony formation of lymphocytes in co‐culture
Author(s) -
Matsuda Shuichi,
Nakao Yoshinobu,
Ohigashi Hajime,
Koshimizu Koichi,
Ito Yohei
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910380613
Subject(s) - diterpene , potency , receptor , biology , microbiology and biotechnology , in vitro , chemistry , immunology , biochemistry
The addition to culture dishes of 10–50 ng/ml of the essential diterpene ester of Sapium sebiferum , 12‐ O ‐hexadecanoylphorbol‐13‐acetate (HPA), increased colony formation of normal peripheral blood lymphocytes co‐cultured with γ‐irradiated HTLV‐1‐producing HUT102 cells. The cells in the stimulated colonies showed an approximately 3‐fold increase in the expression of interleukin‐2 (IL‐2) receptors and a 1.5‐to 2.0‐fold increase in human T‐lymphotropic virus type‐I (HTLV‐I) p19‐positive cells. This biological potency was analogous to that induced by the most potent tumor promoter 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA) and stronger than that of 12‐ O ‐hexade‐canoyl‐16‐hydroxyphorbol‐13‐acetate (HHPA) isolated from Aleurites fordii .