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Lymphoblastoid cell lines and burkitt‐lymphoma‐derivee cell lines differ in the expression of a second epstein‐barr virus encoded nuclear antigen
Author(s) -
Ernberg Ingemar,
Kallin Bengt,
Dillner Joakim,
Falk Kerstin,
EhlinHenriksson Barbro,
Hammarskjöld MarieLouise,
Klein George
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910380517
Subject(s) - epstein–barr virus , biology , virology , burkitt's lymphoma , virus , cell culture , lymphoma , antigen , microbiology and biotechnology , immunofluorescence , lymphoblast , herpesviridae , antibody , genetics , immunology , viral disease
Abstract Twenty‐seven Epstein‐Barr virus (EBV)‐transformed lymphoblastoid cell lines and 27 EBV‐carrying Bur‐kitt‐lymphoma‐derived lines were analyzed for expression of the second EBV‐encoded nuclear antigen (EBNA‐2) by immunoblotting and anticomple‐ment immunofluorescence with EBNA‐2‐specific sera. While all lymphoblastoid cell lines expressed EBNA‐2, only 10 of the 27 BL lines were EBNA‐2‐positive. Comparison of the EBNA‐2 coding Bam HI W‐Y‐ and H‐fragments of EBV‐DNA in the different cell lines by restriction enzyme analysis suggests that EBNA‐2 negativity is due either to sequence diversity or to a deletion in the BamHI WYH region.