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Rearranged HPV 16 molecules in an anal and in a laryngeal carcinoma
Author(s) -
Scheurlen Wolfram,
Stremlau Armin,
Gissmann Lutz,
Höhn Dieter,
Zenner HansPeter,
Hausen Harald Zur
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910380509
Subject(s) - orfs , biology , dna , genome , open reading frame , gene duplication , carcinoma , virology , plasmid , microbiology and biotechnology , virus , genetics , gene , peptide sequence
By hybridization under stringent conditions, one out of two anal carcinomas and one out of 36 laryngeal carcinomas were shown to harbor HPV 16 DNA in high copy number. Further analysis of both tumor DNAs indicated a rearrangement of the viral DNA in the tumor cells. HPV 16 DNA in the anal carcinoma could chiefly be found episomally in two different forms: (1) a minority as 7.9‐kb oligomeric episomes with no apparent modifications; (2) as 10.7‐kb rearranged oligomeric episomes with a duplication of the part of the viral genome encoding the open reading frames (ORF) E 7 , E 1 and parts of E 6 and E 2 . In the laryngeal carcinoma, integrated and episomal HPV 16 DNA molecules of 7.9 kb were present, together with rearranged molecules of approximately 18 kb with multiple duplications of the ORF E 4 and parts of the ORFs E 2 , E 5 , L 1 and L 2 . Possible consequences for transcription of the modified viral genomes are discussed.