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Hras1‐selected, chromosome‐mediated transformants vary in phenotype in vitro and tumorigenic potential in vivo
Author(s) -
Porteous D. J.,
Morten J. E. N.,
Foster M. E.,
Cranston G.,
WeirThompson E.,
Busuttil A.,
Bostock C. J.,
Steel C. M.
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910380422
Subject(s) - in vivo , in vitro , biology , chromosome , genetics , microbiology and biotechnology , cancer research , gene
Transfection of mouse C127 cells with mitotic chromosomes isolated from a human EJ bladder carcinoma cell line gave rise, at high frequency, to foci of transformed cells. Independent, HRAS 1‐selected chromosome‐mediated transformants displayed distinctive cellular morphologies in monolayer culture and colony‐forming abilities in low‐melting‐point agarose. Subcutaneous inoculation of neonatally thymec‐tomized, Ara‐C‐protected, total‐body‐irradiated CBA mice was used to compare the tumorigenic potential of each transformant. Significant quantitative and qualitative differences in tumorigenicity were found between transformants which correlated with differences in malignant phenotype observed in vitro. The sensitivity of the tumorigenicity assay is such that rare transformation events can be selected directly in vivo .