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Recombinant human interferon gamma suppresses HTLV‐III replication in vitro
Author(s) -
Nakashima Hideki,
Yoshida Tsutomu,
Harada Shinji,
Yamamoto Naoki
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910380320
Subject(s) - cytopathic effect , incubation , in vitro , viral replication , virus , interferon , biology , virology , interferon gamma , antigen , microbiology and biotechnology , cell culture , immunology , biochemistry , genetics
Effect of human interferon gamma (rINF‐γ) on HTLV‐III replication was evaluated quantitatively via a novel infection system using HTLV‐I‐carrying MT‐4 cells. Treatment of HTLV‐III‐infected MT‐4 cells with different concentrations (1–1,000 U/ml) of rINF‐γ, which did not affect the growth or viability of uninfected cells, significantly blocked the appearance of immunofluorescent antigens of HTLV‐III and the virus‐induced cytopathic effect in a dose‐dependent manner. A plaque assay was applied to measure the exact amount of viral particles released from HTLV‐III‐infected MT‐4 cultures either untreated or treated with rINFγ after infection. The number of plaques per dish decreased with increasing drug concentrations. About 50% and 80% of HTLV‐III replication were inhibited by the addition of 100 and 1,000 U/ml of rINF‐γ, respectively. The effects of INF were observed by day 5 of incubation with the chemical. However, longer treatment of cells with rINFγ permitted a gradual increase in viral replication. Re‐addition of fresh INF into cultures did not change this pattern significantly.