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Characterization of the platelet‐aggregating activity of cancer cells with different metastatic potential
Author(s) -
Grignani Guido,
Pacchiarini Lucia,
Almasio Piero,
Pagliarino Mauro,
Gamba Gabriella,
Rizzo Salvatore Carlo,
Ascari Edoardo
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910380214
Subject(s) - platelet , chemistry , in vivo , apyrase , neuraminidase , platelet activation , secretion , biochemistry , immunology , biology , enzyme , microbiology and biotechnology
We studied the mechanisms of platelet activation by sublines exhibiting different metastatic potential of two murine experimental tumors: sublines M 4 and M 9 of the benzopyrene‐induced mFS6 sarcoma and sub‐ lines B77‐AA6 and B77–3T3 of RSV‐transformed BALB/c 3T3 fibroblasts. The neoplastic cells of both models induced platelet aggregation, secretion and prosta‐glandin biosynthesis. In the first model but not in the second, all these processes correlated with the in vivo malignancy of cells. Pretreatment of B77‐AA6 and B77–3T3 cells with apyrase significantly decreased platelet aggregation, while pretreatment of M 4 cells was ineffective. However, pretreatment with trypsin or neuraminidase was effective in reducing platelet aggregation induced by M 4 cells, but not that induced by any of the others; furthermore, phospholipase A 2 reduced the platelet response by all sublines. Finally, platelet‐activating activity was also found in the pellets obtained following centrifugation of culture media. These results suggest that platelets are stimulated by cancer cells through different mechanisms; platelet activation by a sialo‐lipo‐protein complex of the cellular membrane was found to be characteristic of the model in which the platelet‐aggregating activity of neoplastic cells correlated with their in vivo metastatic behavior.