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Ca 2+ and calmodulin are involved in the processes conferring stability to DNA in proliferating neoplastic cells
Author(s) -
Lönn Ulf,
Lönn Sigrid
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910370615
Subject(s) - calmodulin , dna , dna fragmentation , fragmentation (computing) , egta , microbiology and biotechnology , biology , dna synthesis , calmodulin binding proteins , biochemistry , chemistry , calcium , enzyme , apoptosis , programmed cell death , ecology , organic chemistry
We have examined, in proliferating neoplastic cells, the effect on DNA of EGTA (a chelator of Ca 2+ ) and W7 (an inhibitor of calmodulin). The treatment results in release of single‐stranded DNA fragments (2‐10 kb) from pre‐labelled HMW DNA. When DNA from synchronized neoplastic cells in S phase is examined, almost all pre‐labelled DNA appears as short fragments. However, fragmentation does not occur in growtharrested cells or in normal cells. Furthermore, fragmentation can be prevented by incubating cells in excessive amounts of Ca 2+ . Hence Ca 2+ and the Ca 2+ ‐binding protein calmodulin are involved in the processes conferring stability to DNA in proliferating neoplastic cells.