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Use of monoclonal antibodies to human breast‐tumor‐associated antigens in fine‐needle aspirate cytology
Author(s) -
Nuti M.,
Mottolese M.,
Viora M.,
Donnorso R. Perrone,
Schlom J.,
Natali P. G.
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910370404
Subject(s) - monoclonal antibody , immunohistochemistry , cytology , pathology , antigen , medicine , breast cancer , fine needle aspiration cytology , mammary gland , monoclonal , antibody , fine needle aspiration , cancer , biopsy , immunology
Fine‐needle aspiration cytology (FNAC) is currently used in evaluating the nature of breast nodules. In the present study we have examined whether monoclonal antibodies (MAbs) to breast‐tumor‐associated antigens (BTAA) can be employed in FNAC for the diagnosis of breast cancer. For this purpose we have used 2 murine MAbs recognizing 2 distinct BTAA expressed by breast tumors, irrespective of their histotype, in an indirect avidin‐biotin immunohistochemical technique on aspirate smears. The results of this study show that, while benign lesions are consistently negative, tumor cells containing aspirates are reactive with at least one MAb in 95% of the cases. Thus, selected MAbs to BTAA may be a powerful diagnostic tool in conjunction with conventional cytology, and because of the objective interpretation of the immunohistochemical findings, FNAC of the breast can eventually be extended outside specialized institutions.