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Multistep process of neoplastic transformation of normal human fibroblasts by 60 CO gamma rays and harvey sarcoma viruses
Author(s) -
Namba Masayoshi,
Nishitani Koji,
Fukushima Fujiko,
Kimoto Tetsuo,
Nose Kiyoshi
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910370314
Subject(s) - biology , oncogene , population , sarcoma , cell culture , neoplastic transformation , cell , malignant transformation , cancer research , microbiology and biotechnology , fibroblast , pathology , virology , carcinogenesis , cancer , genetics , medicine , cell cycle , environmental health
As reported previously (Namba et al. , 1985), normal human fibroblasts were transformed by 60 Co gamma‐ray irradiation into immortal cells with abnormal karyotypes. These transformed cells (KMST‐6), however, showed a low cloning efficiency in soft agar and no transplantability. However, upon treatment with Harvey murine sarcoma virus (Ha‐MSV), the cells acquired elevated clonability in soft agar and transplantability in nude mice. Ha‐MSV alone, however, did not convert normal human fibroblasts into either immortal or tumorigenic cells. The Ha‐MSV‐transformed KMST‐6 cells showed an enhanced expression of the ras oncogene, but normal and 60 Co gamma‐ray‐transformed cells did not. Our current data suggest that gamma rays worked against normal human cells as an initiator, giving rise to chromosome aberrations and immortality, and that Ha‐MSV, probably through its ras oncogene, played a role in the progression of the malignant cell population to a more malignant one showing enhanced colony formation in soft agar and tumorigenicity in nude mice.