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Media conditioned by human leukemic T‐cells induce expression of IL2 receptors and proliferation of normal T lymphocytes
Author(s) -
Kosmatopoulos Constantinos,
Allouche Michèle,
Triebel Frédéric,
Zanti Maria,
Clemenceau Corine,
Gluckman JeanClaude,
Jasmin Claude,
Georgoulias Vassilis
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910370213
Subject(s) - t cell , biology , peripheral blood mononuclear cell , stimulation , receptor , t lymphocyte , il 2 receptor , microbiology and biotechnology , cell growth , monoclonal antibody , interleukin 21 , immunology , antibody , immune system , endocrinology , biochemistry , in vitro
Peripheral blood T‐colony‐forming cells (T‐CFC) from patients with T‐cell acute lymphoblastic leukemias (T‐ALL) and T‐cell non‐Hodgkin lymphomas (T‐NHL) can generate colonies in methylcellulose in the absence of added growth factors and/or mitogenic stimulation. In the present study, we show that media conditioned (LCM) by unstimulated mononuclear cells (MNC) from these patients can induce proliferation (proliferating inducing activity; PIA) and promote colony growth (T‐cell colony promoting activity; T‐CPA) of normal T lymphocytes in the absence of any other mitogenic stimulation. Preincubation of normal E + lymphocytes with some TCPA + , PIA − ‐LCM for 48 hr leads to IL2‐induced cell proliferation in the absence of any other stimulation. Moreover, staining of the cells with anti‐Tac monoclonal antibody (Mab) reveal 9%‐26% Tac + cells. Both PIA and IL2 receptor‐inducing activities were abrogated by treatment of LCM with proteolytic enzymes or by heating at 47°C for 30 min. Modulation of the T3 molecule by OKT3 MAb on normal E + cells did not abrogate the capacity of LCM to induce expression of IL2‐receptors, suggesting that this activity was not mediated by triggering the Ti‐T3 molecular complex. These activities were detected in media conditioned by both unfractionated MNC and blast‐enriched cell fractions, and their production required DNA and RNA synthesis by actively dividing cells. Taken together, these findings indicate that human leukemic T cells spontaneously release activites which can activate normal resting T lymphocytes.