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Altered growth properties and cell surface changes in ras transformed mouse bladder epithelium
Author(s) -
Summerhayes I. C.,
Malone P.,
Visvanathan K.
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910370211
Subject(s) - transfection , urothelium , cell culture , immunofluorescence , epithelium , oncogene , cell , biology , microbiology and biotechnology , urothelial cell , cell growth , cancer research , antibody , immunology , cell cycle , endocrinology , biochemistry , genetics , urinary system
Transfection of the c‐Ha‐ ras ‐1 oncogene, cloned from EJ/T24 cells, into different mouse bladder epithelial cell lines resulted in the acquisition of tumorigenic potential and, in all but one cell line (MB331), anchorage‐independent growth. Sera from syngeneic mice bearing tumours immunoprecipitated an 18 kDa protein from ras ‐transfected urothelial cells which was not detectable in their parental counterparts. Screening of a limited panel of mouse cell lines showed this protein to be urothelium‐specific and associated with the expression of an activated ras gene. Polyoma middle T and v‐ myc ‐transfected bladder epithelial cells did not express this 18kDa protein. Localization of this protein to the cell surface was demonstrated by immunofluorescence and absorption studies.