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The Epstein‐Barr virus determined nuclear antigen is composed of at least three different anitgens
Author(s) -
Dillner Joakim,
Kallin Bengt,
EhlinHenriksson Barbro,
Rymo Lars,
Henle Werner,
Henle Gertrude,
Klein George
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910370205
Subject(s) - antigen , epstein–barr virus , immunofluorescence , virus , mononucleosis , antibody , virology , biology , nuclear protein , cell nucleus , microbiology and biotechnology , indirect immunofluorescence , gammaherpesvirinae , staining , herpesviridae , immunology , viral disease , gene , genetics , transcription factor
Abstract The EBV‐determined nuclear antigen, EBNA, is the only known viral product to be regularly detected in all EBV‐transformed cells. The anticomplement immunofluorescence (ACIF) staining detects an EBV‐specific nuclear reaction that has recently been shown to be due to at least 2 different proteins, EBNA‐1 and EBNA‐2, encoded by different parts of the viral genome. We now report the existence of a third antigen of the EBNA complex, designated as EBNA‐3. Serum from a patient with chronic infectious mononucleosis contained no detectable antibodies to EBNA‐1 and had only a low EBNA‐2 antibody level. Nevertheless, it gave an EBV‐specific nuclear reaction of normal intensity and stained EBNA‐2‐positive and EBNA‐2‐negative EBV‐carrying lines equally well. Immunoblotting with the same serum identified a new EBV‐specific nuclear protein of 143‐157 kDa.