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Actin filaments and tumorigenicity in a fischer rat embryo fibroblast cell line (3Y1) transformed by ultraviolet‐irradiated HSV
Author(s) -
Tsuchie H.,
Katsumoto T.,
Hama S.,
Hattori N.,
Morimoto H.,
Kamahora T.,
Kurimura T.
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910370125
Subject(s) - fibroblast , actin , immunofluorescence , cell culture , embryo , microbiology and biotechnology , biology , cell , cytoskeleton , chemistry , antibody , immunology , biochemistry , genetics
The characteristics of the cytoskeleton of a Fischer rat embryo fibroblast cell line (3Y1) transformed by ultraviolet (UV)‐irradiated HSV were studied by indirect immunofluorescence using anti‐actin IgG. Parental 3Y1 cells possessed well‐developed actin filaments, while 3Y1 cells transformed by UV‐irradiated HSV also retained well‐developed actin filaments. Transformed cells were divided into 2 groups according to tumorigenicity in newborn Fischer rats; one had a strongly tumorigenic potential and the other a weakly tumorigenic potential. Tumor‐derived cell lines exhibited a highly tumorigenic potential, and were also divided into 2 groups, one with well‐developed actin filaments and the other without well‐developed actin filaments. Our results suggested that transformation or tumor formation by HSV is a multi‐step process and that morphological loss of actin filaments in the cells is not essential to the tumorigenic potential of the cells transformed by HSV.