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Molecular cloning and characterization of the dna of a new human papillomavirus (hpv 30) from a laryngeal carcinoma
Author(s) -
Kahn Tomas,
Schwarz Elisabeth,
Hausen Harald Zur
Publication year - 1986
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910370111
Subject(s) - clone (java method) , biology , dna , dna–dna hybridization , molecular cloning , microbiology and biotechnology , virology , carcinoma , hybridization probe , gene , dna sequencing , nucleic acid thermodynamics , cloning (programming) , sequence analysis , genetics , base sequence , peptide sequence , computer science , programming language
DNA from a human laryngeal carcinoma was molecularly cloned in Lambda L47. The gene library was screened for human papillomavirus (HPV)‐related sequences by hybridization analysis with 32 P‐labelled HPV 16 DNA at conditions of low stringency (Tm −40°C). One of the clones (4‐5) with an insert of 7.8 kb showed cross‐hybridization with most of the known HPV types (Tm −40°C), and with several of them even under more stringent conditions (Tm ‐30°C). No signal was detected under high‐stringency conditions (Tm −20°C). The co‐linear alignment of clone 4‐5 with HPV 16 DNA could be demonstrated by hybridization experiments and also by partial DNA sequence analysis. We conclude that clone 4‐5 represents a new HPV type tentatively designated HPV 30. HPV 30 DNA was also detected in 2 genital lesions but not in 41 laryngeal carcinomas analyzed so far. Its presence in other tumor DNA is now under investigation.