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DBA/2‐like minor histocompatibility antigens on a BALB/c lymphoma. A balb/c anti‐DBA/2 serum which lyses the tumor and blocks balb/c anti‐tumor and anti‐DBA/2 effectors
Author(s) -
Ballinari Dario,
Grazioli Laura,
Sensi Maria Luisa,
Parmiani Giorgio,
Borrello Maria Grazia
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910360516
Subject(s) - antigen , cytotoxic t cell , ctl* , balb/c , antiserum , minor histocompatibility antigen , pan t antigens , histocompatibility , cytotoxicity , microbiology and biotechnology , biology , immunology , lymphoma , major histocompatibility complex , virology , human leukocyte antigen , immune system , cd8 , in vitro , biochemistry
We have previously shown that BALB/c anti‐DBA/2 T cells can lyse the Moloney virus‐induced BALB/c lymphoma YC8. In order to determine whether serologically defined minor histocompatibility antigens (MiHA) cross‐reacting with those of DBA/2 tissues are present on YC8, we produced an antiserum directed against non‐H‐2 antigens by immunizing BALB/c mice with DBA/2 Con A and lipopolysaccharide (LPS)‐induced lymphoblasts. In a direct complement‐dependent cytotoxicity assay, the antiserum (OR‐I) lysed DBA/2 and YC8 but not BALB/c lymphocytes and blasts. No reactions against viral antigens were detected in the antisera as shown by the lack of cytotoxicity on a panel of lymphomas expressing a variety of viral antigens. In addition, OR‐I was able to specifically block a cytotoxic T lymphocyte (CTL), H‐2‐restricted BALB/c anti‐DBA/2 cytotoxic response when bound to DBA/2 or to YC8 target cells. These results indicate that antigens cross‐reacting between YC8 lymphoma and DBA/2 tissues are serologically defined MiHA of DBA/2 background and that OR‐I serum can block a CTL reaction by binding to target antigen rather than to major histocompatibility complex products.