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Papillomavirus DNA in human tongue carcinomas
Author(s) -
De Villiers E.M.,
Weidauer H.,
Otto H.,
Zur Hausen H.
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910360510
Subject(s) - papilloma , southern blot , dna , tongue , biology , pathology , human papilloma virus , dot blot , papillomaviridae , nucleic acid thermodynamics , virus , virology , microbiology and biotechnology , cancer , genetics , medicine , base sequence , cervical cancer
Seven biopsies from different carcinomas of the tongue were analyzed for human papilloma virus (HPV) sequences by Southern blot analysis. After hybridization with various types of human papilloma viruses, 1 tumors were found to be positive. Whereas DNA from one tumor hybridized with HPY 2 DNA under conditions of high stringency, the 2 other positive biopsies contained HPV 16 DNA. All positive tumors revealed high copy numbers of the respective DNA. The cleavage pattern of the HPV‐2‐positive tumor differed from the established HPV 2 prototypes. Minor differences from the HPV 16 prototype were also noted in one of the HPV‐16‐positive tongue carcinomas.