Premium
Partial purification and characterization of a vascular permeability factor secreted by a human colon adenocarcinoma cell line
Author(s) -
Lobb Roy R.,
Key Marc E.,
Alderman Edward M.,
Fett James W.
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910360410
Subject(s) - histamine , trypsin , vascular permeability , cell culture , chemistry , biochemistry , proteases , in vitro , biology , microbiology and biotechnology , endocrinology , enzyme , genetics
Abstract The established human colon adenocarcinoma cell line, HT‐29, secretes a vascular permeability factor (VPF) in vitro . The factor has been purified from serum‐free conditioned medium by acidification, cation‐exchange, and reverse‐phase and anion‐exchange high‐performance liquid chromatography. The VPF is a non‐glycosylated acidic protein of apparent molecular weight 45,000, and is at least 4 orders of magnitude more potent than histamine as an inducer of vascular permeability. Its biological activity is unaffected by soybean trypsin inhibitor or by inhibitors of histamine, kinins, prostaglandins, or acid proteases. The VPF induces vascular permeability within minutes, which suggests a direct effect on the endothelial cell.