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The establishment and characterization of a new balb/c angiosarcoma tumor system
Author(s) -
Zvibel Isabel,
Raz Avraham
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910360220
Subject(s) - cell culture , biology , cell , clone (java method) , vinculin , pathology , cytoskeleton , microbiology and biotechnology , cancer research , genetics , medicine , dna
Abstract A BALB‐3T3/A31 untransformed cloned cell line and 3 selected variants derived from this parental cell line, expressing 3 increasingly malignant phenotypes, have been established and characterized in vivo and in culture. This new tumor series, identified as angiosarcoma, consists of an anchorage‐independent nontumorigenic cell clone and 2 sublines exhibiting tumorigenic and metastatic properties. Morphological examination revealed that the 3 transformed cell variants differed from the normal parental cells and were not contact‐inhibited. Karyotype and rate of cell proliferation in culture were similar for all the cell variants. Cytoskeletal visualization by immunofluorescence staining revealed that the tumorigenic and metastatic cell lines expressed an altered organization of actin cables and a smaller number of vinculin‐containing focal contacts. Lactoperoxidase iodination of cell surface proteins showed the appearance of an M γ 86,000 protein in the tumorigenic and metastatic cell variants. Analysis of cell‐surface glycoproteins demonstrated an increased sialylation of M γ 66,000 and M γ 62,000 glycoproteins in the transformed, tumorigenic and metastatic cell lines. This angiosarcoma tumor model system allows investigation of cellular characteristics which might be relevant to specific stages in tumor progression.