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Production of PDGF‐like growth factor by breast cancer cell lines
Author(s) -
Rozengurt Enrique,
SinnettSmith James,
TaylorPapadimitriou Joyce
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910360218
Subject(s) - platelet derived growth factor receptor , growth factor , platelet derived growth factor , stromal cell , cell culture , cancer research , biology , cell growth , microbiology and biotechnology , breast cancer , cancer , endocrinology , medicine , biochemistry , receptor , genetics
The breast cancer cell line T47D produces factors which show mitogenic activity for 3T3 cells. Here we describe the partial purification of one of these factors and show that it has properties similar to plateletderived growth factor (PDGF). The T47D factor is a heat‐stable hydrophobic protein with a molecular weight of around 30,000, which inhibits the binding of 125 I‐EGF in a temperature‐dependent manner. This 30 kd protein does not act synergistically with PDGF or fibroblast‐derived growth factor (FDGF; also PDGF‐like) in stimulating DNA synthesis; moreover, like these two factors, its mitogenic activity can be inhibited by an antiserum raised against human PDGF. A PDGF‐like growth factor was also found in the serum free medium of the breast cancer cell line MDA‐MB157. Since PDGF acts on mesenchymal cells, the production of PDGF like growth factors by breast cancer cells suggests a basis for the intense stromal reaction seen in human breast cancers.