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N‐ras gene activation in the rd human rhabdomyosarcoma cell line
Author(s) -
Chardin Pierre,
Yeramian Patrick,
Madaule Pascal,
Tavitian Armand
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910350513
Subject(s) - rhabdomyosarcoma , gene , cancer research , cell culture , biology , medicine , genetics , pathology , sarcoma
A transforming N‐ ras allele was molecularly cloned from the RD human rhabdomyosarcoma cell line, and the nature of its activation studied. Construction of chimeric recombinants between the RD‐transforming allele and a normal human allele enabled us to localize the alteration responsible for the activation to the second exon of the N‐ ras gene. The nucleotide sequence of this exon, when compared to that of the normal allele, revealed a single difference at the 61st amino acid position of the encoded protein; the CAA codon for glutamine in the normal allele was mutated to a CAT codon for histidine in the RD‐transforming allele. This result is the first description of a histidine replacing glutamine in the 61st position and provides further evidence that the 61st amino acid is one of the preferential sites for N‐ ras activation.