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Monoclonal antibody production to a B16 melanoma associated antigen
Author(s) -
Tomita Yasushi,
Montague Paul M.,
Hearing Vincent J.
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910350419
Subject(s) - antigen , epitope , monoclonal antibody , antibody , biology , microbiology and biotechnology , albumin , immunology , biochemistry
Abstract Eight different rat hybridoma cell lines have been produced which secrete MAbs Abbreviations: BSA, bovine serum albumin; ELISA, enzymelinked immunosorbent assay; MSA, murine serum albumin; PBS, phosphate‐buffered saline; MAb, monoclonal antibody; s.c., subcutaneous. identifying the melanoma‐specific B700 antigen. This antigen has been previously shown to have significant sequence homology to the mammalian serum albumins, but antibodies specifically raised against B700 cross‐react only with murine albumin. Each of these MAbs has been shown by Western immunoblotting to recognize an independent epitope on the B700 protein; it is concluded not only that the B700 protein is a highly immunogenic antigen, but also that the sequence homologies between the B700 antigen and murine albumin must occur extensively throughout the molecule.