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Inhibition of growth of certain human tumour cell lines by a factor derived from human fibroblast‐like cell lines. I. Demonstration by mixed culture and by use of cell washings
Author(s) -
Wu KeFu,
Pope John H.,
Ellem Kay A. O.
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910350410
Subject(s) - cell culture , fibroblast , pronase , biology , cell growth , cell , lymphoblast , microbiology and biotechnology , biochemistry , genetics , trypsin , enzyme
The growth of 7/9 Burkitt's lymphoma (BL) cell lines and 5/6 human melanoma cell lines was inhibited by certain human fibroblast‐like cell lines, especially human myofibroblast cell lines. In contrast to BL lines, 3/5 lymphoblastoid cell lines were less susceptible and 3/4 human leukaemic lines were resistant. The effect was time‐ and concentration‐dependent. Co‐cultivation led to inhibition of tumour‐cell growth within 3 days, but after removal from the fibroblasts, washing, and resuspending in fresh medium some tumour cells recovered and growth improved. The inhibitory activity was also demonstrated with washings of fibroblast‐like cell lines, and was sensitive to pronase. The biological significance of this factor remains to be defined.