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Natural killer cell resistance in K‐562 cell sublines
Author(s) -
Foster J. S.,
Wust C. J.,
Lozzio C. B.,
Bamberger E. G.,
Ichiki A. T.
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910350310
Subject(s) - clone (java method) , biology , lysis , natural killer cell , cytolysis , microbiology and biotechnology , cell culture , lytic cycle , neuraminidase , cell , antibody , lymphokine activated killer cell , interleukin 21 , cytotoxicity , immunology , in vitro , t cell , immune system , gene , biochemistry , genetics , virus
Sublines of the hematopoietic stem cell line K‐562 were tested for their susceptibility to human natural killer (NK) cell activity. A correlation was found between the degree of NK‐mediated lysis and the presence or absence of particular chromosomal markers. K‐562 sublines susceptible to lysis by NK were found to express karyotypically a deletion 9‐ and a marker 8(tl : 18), whereas resistant sublines did not express these markers. A cloned K‐562 subline B I V was chosen as representative of a resistant subline. This subline was resistant to lysis even after prolonged incubation and activation of the NK cells with interferon. However, it was found that B I V was lysed by both antibody‐dependent, complement‐mediated and antibody‐dependent cellular mechanisms at levels comparable to those seen with NK‐sensitive K‐562 sublines. Subline B I V did compete poorly with NK‐sensitive K‐562 in cold‐target inhibition; however, conjugate‐formation assays demonstrated that the binding of NK cells to B I V cells is comparable to that of NK‐sensitive K‐562 cells. We suggest that cells of the cloned line B I V are recognized normally by NK cells but do not activate the lytic mechanism of the bound NK cell. Treatment of the resistant clone B I V with neura minidase did not lead to enhanced levels of lysis. Protein extracts of NK‐sensitive K‐562 sublines efficiently inhibited lysis by NK cells but extracts of the resistant clone, B I V, did not inhibit lysis, suggesting that the clone lacks cell surface determinants involved in the post‐recognitive activation of the NK cytolytic mechanism.