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Capacity of B‐lymphocytic lines of diverse tumor origin to produce and respond to B‐cell growth factors: A progression model for B‐cell lymphomagenesis
Author(s) -
Gordon John,
Åman Pierre,
Rosén Anders,
Ernberg Ingemar,
EhlinHenriksson Barbro,
Klein George
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910350218
Subject(s) - b cell , cell culture , biology , lymphoma , cancer research , cell growth , lymphoblast , burkitt's lymphoma , immunology , antibody , genetics
Abstract Human cell lines established from cases of acute lymphoblastic leukemia, lymphosarcoma, Burkitt's lymphoma and multiple myeloma and representing stages of B‐lymphocyte development ranging from pre‐B through to plasma cells, were assessed for their ability to produce and respond to B‐cell growth factors (BCGF). All B‐cell lines studied were found to be constitutive producers of a growth activity which assisted the S‐phase entry of normal activated B‐cells and provided growth support for lymphoblastoid cells transformed by Epstein‐Barr virus. Furthermore, all lines responded by enhanced proliferation to supernatants from a BCGF‐producing T‐cell hybridoma. Not all lines, however, displayed autostimulation to their own supernatants and no tumor B‐cell line appeared totally dependent on soluble factors for its growth. Non‐tumorigenic B‐cell lines, by contrast, revealed a strict dependency on homologous growith factor for their continued proliferation in suspension culture. The findings support a progression model of lymphomagenesis based upon the utilization, production and, ultimately, emancipation from growth‐promoting soluble factors.