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Changes in fibronectin synthesis and binding distribution in SV40‐transformed human keratinocytes
Author(s) -
Edelman B.,
Steinberg M. L.,
Defendi V.
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910350213
Subject(s) - fibronectin , immunofluorescence , microbiology and biotechnology , transformation (genetics) , cell , biology , cell culture , keratinocyte , chemistry , immunology , biochemistry , antibody , genetics , gene
Abstract We have studied the synthesis and distribution of fibronectin in human epidermal keratinocytes infected by SV40, a system in which the acquisition of transformed properties occurs in a sequential and progressive manner. Immunofluorescence studies showed that cultured unifected keratinocytes do not exhibit fibronectin on the superficial cell surface, but that virus‐infected cells come to display superficial fibronectin‐containing cables in a density‐dependent manner after a certain point in the transformation process. In contrast, organized arrays of fibronectin‐containing fibrils associated with the cell‐substrate attachment complex were seen in uninfected keratinocytes and in virus‐infected cells at all stages of the transformation process. Studies of fibronectin synthesis using metabolic labelling of cell proteins with 35 S methionine showed that viral infection caused a striking increase in overall fibronectin synthesis, although with a much higher proportion of newly synthesized fibronectin being secreted into the cell culture medium than in the case of the uninfected cells.