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The use of hybridoma cells as a murine model to study tumor immunity
Author(s) -
Ezaki Taichi,
Marbrook John
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910350117
Subject(s) - cytotoxic t cell , spleen , immune system , biology , immunity , tumor cells , antigen , antibody , in vivo , immunology , hybridoma technology , monoclonal antibody , in vitro , microbiology and biotechnology , cancer research , genetics
The secretion of antibody by hybridoma cells allows the growth of individual cells to be measured by a hemolytic plaque assay. Similarly, plaque reduction assays are sensitive indicators of tumor immunity. A series of strains of hybridoma have been isolated from an original isolate which is sensitive to cytotoxic T cells and NK cells. In the derivation of a spleen‐seeking variant, the cells appear to have lost immunogenic but not antigenic characteristics. This model lends itself to the quantitative study of immune constraints on tumor growth in vivo. Combined with the selection of tumor variants, the model is of wide application to many fields of tumor biology, particularly where sensitive measurement of tumor cell number and viability is crucial.