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Establishment of the amsalem T‐cell line from a patient with acute lymphoblastic leukemia. Expression of E‐receptor‐associated antigens in cells incapable of forming E‐rosettes
Author(s) -
Bassat H. Ben,
Rabinowitz R.,
Polliack A.,
Hadar R.,
Schlesinger M.
Publication year - 1985
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910350106
Subject(s) - antigen , monoclonal antibody , antibody , cytotoxic t cell , microbiology and biotechnology , receptor , antiserum , biology , leukemia , t cell , cell culture , immunology , immune system , in vitro , biochemistry , genetics
A new T‐cell line (Amsalem) was established from the peripheral blood of a patient with pre‐T leukemia. Amsalem cells are unique in that they possess antigenic determinants associated with the E‐receptor, yet fail to form rosettes with sheep red blood cells (SRBC). Amsalem cells were found to possess morphological and cytochemical features characteristic of T‐lymphocytes, and were sensitive to the cytotoxic effect of rabbit antisera specific for T‐cell antigens. In immunofluorescent tests with monoclonal antibodies, Amsalem cells showed a strong reactivity with the OKT‐11A and A‐22 antibodies, specific for the E‐receptor. The cells were reactive with OKT‐4 and showed a very weak reactivity with OKT‐6 and OKT‐8. No reactivity was found with the OKT‐3, Leu 7, Leu II, and OKMI antibodies. Amsalem cells failed to form rosettes with SRBC; however, mouse anti‐Amsalem serum inhibited the formation of E‐rosettes. It is concluded that the Amsalem cell line is a line of pre‐T leukemia cells characterized by a dissociation between its inability to form E‐rosettes and the presence of antigenic constituents of the E‐receptor.