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Effects of DL‐alpha‐tocopheryl succinate in combination with sodium butyrate and camp stimulating agent on neuroblastoma cells in culture
Author(s) -
Rama Bhola N.,
Prasad Kedar N.
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910340619
Subject(s) - sodium butyrate , butyrate , vitamin , sodium , biochemistry , chemistry , endocrinology , medicine , biology , organic chemistry , fermentation , gene
The effect of dl‐alpha‐tocopheryl (vitamin E) succinate in combination with Prostaglandin A 2 (PGA 2 ) and sodium butyrate on mouse neuroblastoma cells (NBP 2 ) in culture, according to the criteria of growth inhibition and morphological differentiation (neurite formation), was studied. Results showed that PGA 2 and sodium butyrate inhibited the growth of NB cells in a dose‐dependent manner. The combined effects of vitamin E succinate with PGA 2 or sodium butyrate, according to the criterion of growth inhibition, were additive. Vitamin E succinate by itself did not induce morphological differentiation, but it enhanced PGA 2 ‐induced morphological differentiation. Sodium butyrate alone or in combination with vitamin E succinate did not significantly increase the level of morphological differentiation. Sodium succinate and an equal amount of solvent (ethanol) failed to modify the effect of PGA 2 or sodium butyrate. This suggests that the effect of vitamin E succinate in modifying the response of PGA 2 and sodium butyrate on NB cells is due to the effect of vitamin E rather than to that of succinate.