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Cytolytic activity of interferon‐gamma and its synergism with 5‐fluorouracil
Author(s) -
Le Junming,
Yip Yum K.,
Vilček Jan
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910340411
Subject(s) - cytolysis , interferon gamma , cell culture , monoclonal antibody , microbiology and biotechnology , fibroblast , interferon , in vitro , biology , cytotoxicity , antibody , cytokine , virology , immunology , biochemistry , genetics
Abstract Highly purified natural or recombinant human immune interferon (IFN‐gamma) was found to be directly cytolytic to certain tumor cell lines in vitro . Out of 5 human tumor cell lines and one normal fibroblast line tested, the colon adenocarcinoma line HT‐29 and the rhabdomyosarcoma line A673 were highly sensitive to cytolysis by interferon, as determined by 125I‐iododeoxyuridine release in a 72 h microcytotoxicity assay. Cytolysis was marked at IFN‐gamma concentrations of less than I U/ml, and it reached a near‐maximal level at 6.4 U/ml. A synergistic cytolysis on HT‐29 cells of IFN‐gamma and 5‐fluorouracil (5‐FU) was observed at 5‐FU concentrations ranging from 64 to 640 μgl ml. In contrast, no synergism was observed between IFN‐gamma and mitomycin C. The direct cytolytic activity and synergistic cytolysis with 5‐FU of the IFN‐gamma preparations used in the present study were abolished completely by treatment with a neutralizing monoclonal antibody specific for human IFN‐gamma.