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Colony formation in the absence of added growth factors by peripheral blood T‐cell colony‐forming cells of patients with T‐cell malignancies
Author(s) -
Georgoulias Vassilis,
Bourinbaiar Aldar,
Amesland Françoise,
Ca Chantal,
Auclair Huguette,
Jasmin Claude
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910340407
Subject(s) - peripheral blood mononuclear cell , lymphoblast , clonogenic assay , biology , microbiology and biotechnology , cell division , immunology , cell , precursor cell , cell culture , andrology , in vitro , medicine , biochemistry , genetics
Clonogenic cells from peripheral blood of 13/16 patients with T‐cell malignancies generated colonies in methylcellulose in absence of added growth factors or mitogenic stimulation. Spontaneous colonies were also obtained from purified cell fractions (E − OKT3 − and/or E + cells) in 73% and 57% of the patients, respectively. No spontaneous colony growth was observed with mononuclear cells of patients with solid tumors, non‐T‐cell leukemias or normal subjects. Colonies consisted of acid‐phosphatase‐positive, myeloperoxidase‐and PAS‐negative lymphoblasts bearing T‐cell surface markers. Although the phenotype of pooled colony cells from either unfractionated mononuclear cells or E − OKT3 − ‐derived colonies varied from patient to patient, the colonies, like fresh leukemic cells, were mostly composed of relatively immature cells as assessed by the high proportion (>40%) of OKT6 + and OKT10 + cells and the low proportion (<40%) of OKT3 + and/or E + cells. Cytogenetic analysis of colony cells revealed either normal metaphases or chromosome anomalies similar to those observed in fresh leukemic cells. Moreover, cells from primary colonies exhibited a capacity for self‐renewal in the absence of added growth factors.