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Reversibility of the transformed and neoplastic phenotype. IV. Effects of long‐term interferon treatment of C3H/10T1/2 cells transformed by methylcholanthrene and SV40 virus
Author(s) -
BroutyBoye D.,
WybierFranqui J.,
Calvo C.,
Feunteun J.,
Gresser I.
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910340119
Subject(s) - interferon , reversion , biology , methylcholanthrene , virus , phenotype , cell culture , cell , virology , microbiology and biotechnology , gene , genetics , carcinogen
Abstract The effects of long‐term treatment with mouse interferon on the phenotype of untransformed C3H/10TI/2 cells and cloned derivatives transformed by methylcholanthrene (MCA) and Simian virus 40 (SV40) were investigated. Continuous presence of interferon induced morphologic reversion, with the development of thick, submembranous filaments in MCA‐transformed cells, where as no morphological effects were detected in SV40‐transformed cells. Interferon inhibited the proliferation of all three cell lines and maintained low saturation densities. However, prolonged treatment of MCA‐transformed cells with interferon rendered them tolerant toward the antiproliferative and antiviral activities of interferon although 2‐A synthetase activity was induced. Interferon treatment reduced the capacity of both MCA and SV40‐transformed cells to form colonies in agar and decreased the tumorigenicity of MCA‐transformed but not SV40‐transformed cells in mice. These results indicate that the same cell type transformed by different means has different sensitivities to a number of interferon‐induced changes in the cell phenotype.