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Tumor‐cell‐platelet aggregation does not correlate with metastatic potential of rat 13762NF mammary adenocarcinoma tumor cell clones
Author(s) -
Estrada Jaime,
Nicolson Garth L.
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910340118
Subject(s) - clone (java method) , in vivo , platelet , metastasis , adenocarcinoma , in vitro , cell , cell culture , biology , pathology , cancer research , mammary tumor , cancer , immunology , medicine , breast cancer , biochemistry , dna , genetics , microbiology and biotechnology
The ability of tumor cells to induce platelet aggregation has been correlated with their capacities to colonize the lungs of experimental animals. We tested this hypothesis by studying the ability of cloned, low‐passage metastatic tumor cell lines derived from rat 13762NF mammary adenocarcinoma to aggregate rat platelets in vitro and in vivo, and we then compared this activity to metastatic potential by determining the incidence of lung metastasis after subcutaneous or intravenous inoculation of the tumor cell clones into syngeneic rats. Our results failed to show a correlation between in vitro platelet‐aggregating activity and metastatic potential. In this system we could not detect platelet‐aggregation activity with the most metastatic tumor clone, while the least metastatic clone clearly possessed high platelet‐aggregating activity. In addition, by measuring changes in blood platelet and fibrinogen concentrations at various intervals following intravenous injection of tumor cell clones, we were able to confirm in vivo the observed in vitro differences in their plateletaggregating activities. Thus, platelet aggregating activity is heterogeneously expressed among 13762NF cell clones and appears unrelated to spontaneous or experimental metastasis in this tumor system.