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Monoclonal antibody against an antigen selectively assembled into vesicular stomatitis virus virions from HeLa cells
Author(s) -
Poláková Katarina,
Závadová Zuzana,
Závada Jan,
Russ Gustáv
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910340116
Subject(s) - vesicular stomatitis virus , hela , antigen , monoclonal antibody , microbiology and biotechnology , virology , biology , cell fusion , cell culture , antibody , virus , spleen , immunology , genetics
A mouse hybridoma cell line IIB9, secreting IgG2b antibody specific for a HeLa cell antigen, was obtained by fusion of a mouse myeloma cell line with spleen cells from mice immunized with purified VSV tsO45 mutant (defective in assembly of G protein) which had been reproduced at a non‐permissive temperature in HeLa cells. The monoclonal antibody IIB9 was strictly specific for HeLa cells in two tests: (1) reaction with VSV or Chandipura virus phenotypically mixed with host cell antigen, (2) complement‐dependent cytotoxicity test ( 51 Cr‐release).