Monoclonal antibody against an antigen selectively assembled into vesicular stomatitis virus virions from HeLa cells | Zendy

Poláková Katarina | Zendy; Závadová Zuzana | Zendy; Závada Jan | Zendy; Russ Gustáv | Zendy

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Monoclonal antibody against an antigen selectively assembled into vesicular stomatitis virus virions from HeLa cells

Author(s) -

Poláková Katarina,

Závadová Zuzana,

Závada Jan,

Russ Gustáv

Publication year - 1984

Publication title -

international journal of cancer

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.475

H-Index - 234

eISSN - 1097-0215

pISSN - 0020-7136

DOI - 10.1002/ijc.2910340116

Subject(s) - vesicular stomatitis virus , hela , antigen , monoclonal antibody , microbiology and biotechnology , virology , biology , cell fusion , cell culture , antibody , virus , spleen , immunology , genetics

A mouse hybridoma cell line IIB9, secreting IgG2b antibody specific for a HeLa cell antigen, was obtained by fusion of a mouse myeloma cell line with spleen cells from mice immunized with purified VSV tsO45 mutant (defective in assembly of G protein) which had been reproduced at a non‐permissive temperature in HeLa cells. The monoclonal antibody IIB9 was strictly specific for HeLa cells in two tests: (1) reaction with VSV or Chandipura virus phenotypically mixed with host cell antigen, (2) complement‐dependent cytotoxicity test ( 51 Cr‐release).

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