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Transformation of atla‐negative leukocytes by blood components from anti‐ATLA‐positive donors In vitro
Author(s) -
Miyamoto Kanji,
Tomita Noriko,
Ishii Akio,
Nishizaki Takeshi,
Kitajima KoIchi,
Tanaka Toshio,
Nakamura Takashi,
Watanabe Sekiko,
Oda Takuzo
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910330603
Subject(s) - platelet , in vitro , biology , cytoplasm , antigen , virus , whole blood , immunology , antibody , microbiology and biotechnology , virology , chemistry , biochemistry
Anti‐ATLA‐positive blood components transformed healthy human leukocytes in vitro. Blood components examined were packed red cells, whole blood, platelet concentrate and fresh frozen plasma. Leukocytes present in anti‐ATLA‐positive blood components such as packed red cells, whole blood and platelet concentrate easily transformed anti‐ATLA‐negative leukocytes. Co‐culture in fresh frozen plasma, however, did not transform recipient leukocytes, and leukocytes of anti‐ATLA‐positive recipients proved refractory to transformation. The transformed cells were morphologically lymphoid, grew in suspension, and possessed normal recipient karyotypes except in the case of three platelet concentrates. A high proportion of all the transformed populations formed Erosettes with neuraminidase‐treated sheep erytrocytes. The cytoplasm of over 90% of each recipient was stained brilliantly with antibodies against ATLV‐determined antigens. Electron microscopy of these transformed cells revealed many C‐type virus particles in the extracellular space. Blood components, such as packed red cells, whole blood and platelet concentrate, containing leukocytes from anti‐ATLA‐positive donors, should be used cautiously to prevent the transmission on ATLV to anti‐ATLA‐negative recipients.